error prone pcr Search Results


error-prone pcr kits  (Beijing Solarbio Science)
90
Beijing Solarbio Science error-prone pcr kits
Error Prone Pcr Kits, supplied by Beijing Solarbio Science, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/error+prone+pcr/pm40407227-388-1-6?v=Beijing+Solarbio+Science
Average 90 stars, based on 1 article reviews
error-prone pcr kits - by Bioz Stars, 2026-08
90/100 stars
  Buy from Supplier
error prone pcr  (GenScript corporation)
90
GenScript corporation error prone pcr
Principle of in vivo disulfide bond isomerase screen using mutant PhoA*. (A) ① Reduced wt alkaline phosphatase (wt PhoA RED ) with four cysteines ( yellow balls with numbers indicating cysteine residue) is correctly oxidized by DsbA to form two consecutive disulfide bonds. ② Active folded PhoA (wt PhoA OXI ) can hydrolyze XP, ③ resulting in blue colonies. ④ Reduced mutant alkaline phosphatase (PhoA* RED ) lacking the first cysteine (C168A) and with an additional mutant cysteine (S410C in magenta) is mis-oxidized by DsbA (PhoA* MIS-OXI ), resulting in unknown disulfide bond pattern. ⑤ Colonies expressing inactive misfolded PhoA*, which cannot hydrolyze XP, remain their natural white color. ⑥ Misfolded PhoA* is isomerized by DsbC or DsbG* mutants to an active form (PhoA* OXI ) with an unknown disulfide bond pattern resulting in blue colonies. (B) Selection of DsbG* mutants on MOPS-XP plates. ① Cells lacking dsbC , dsbG , phoA and harboring an empty expression vector along with pBAD33-PhoA* are opaque and ecru when plated on the selective MOPS-XP media. ② Cells turn blue when expressing DsbC from the plasmid pDSW204. ③ Cells remain ecru when expressing DsbG from the plasmid pMER79. ④ <t>PCR</t> mutagenized library of pMER79- dsbG *-flag, transformed into cells lacking dsbC , dsbG , phoA , and blue colonies, which have active folded PhoA*, was selected. MOPS, 3-( N -morpholino)propanesulfonic acid; <t>PCR,</t> <t>polymerase</t> chain reaction; wt, wild type; XP, 5-bromo-4-chloro-3-indolyl phosphate.
Error Prone Pcr, supplied by GenScript corporation, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/error+prone+pcr/pmc04624244-235-21-24?v=GenScript+corporation
Average 90 stars, based on 1 article reviews
error prone pcr - by Bioz Stars, 2026-08
90/100 stars
  Buy from Supplier
error-prone pcr  (GenStar Biosolutions)
90
GenStar Biosolutions error-prone pcr
Principle of in vivo disulfide bond isomerase screen using mutant PhoA*. (A) ① Reduced wt alkaline phosphatase (wt PhoA RED ) with four cysteines ( yellow balls with numbers indicating cysteine residue) is correctly oxidized by DsbA to form two consecutive disulfide bonds. ② Active folded PhoA (wt PhoA OXI ) can hydrolyze XP, ③ resulting in blue colonies. ④ Reduced mutant alkaline phosphatase (PhoA* RED ) lacking the first cysteine (C168A) and with an additional mutant cysteine (S410C in magenta) is mis-oxidized by DsbA (PhoA* MIS-OXI ), resulting in unknown disulfide bond pattern. ⑤ Colonies expressing inactive misfolded PhoA*, which cannot hydrolyze XP, remain their natural white color. ⑥ Misfolded PhoA* is isomerized by DsbC or DsbG* mutants to an active form (PhoA* OXI ) with an unknown disulfide bond pattern resulting in blue colonies. (B) Selection of DsbG* mutants on MOPS-XP plates. ① Cells lacking dsbC , dsbG , phoA and harboring an empty expression vector along with pBAD33-PhoA* are opaque and ecru when plated on the selective MOPS-XP media. ② Cells turn blue when expressing DsbC from the plasmid pDSW204. ③ Cells remain ecru when expressing DsbG from the plasmid pMER79. ④ <t>PCR</t> mutagenized library of pMER79- dsbG *-flag, transformed into cells lacking dsbC , dsbG , phoA , and blue colonies, which have active folded PhoA*, was selected. MOPS, 3-( N -morpholino)propanesulfonic acid; <t>PCR,</t> <t>polymerase</t> chain reaction; wt, wild type; XP, 5-bromo-4-chloro-3-indolyl phosphate.
Error Prone Pcr, supplied by GenStar Biosolutions, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/error+prone+pcr/bio_rxiv__2024__12__20__629597-263-5-7?v=GenStar+Biosolutions
Average 90 stars, based on 1 article reviews
error-prone pcr - by Bioz Stars, 2026-08
90/100 stars
  Buy from Supplier
error-prone pcr  (SAMDI Tech)
90
SAMDI Tech error-prone pcr
Principle of in vivo disulfide bond isomerase screen using mutant PhoA*. (A) ① Reduced wt alkaline phosphatase (wt PhoA RED ) with four cysteines ( yellow balls with numbers indicating cysteine residue) is correctly oxidized by DsbA to form two consecutive disulfide bonds. ② Active folded PhoA (wt PhoA OXI ) can hydrolyze XP, ③ resulting in blue colonies. ④ Reduced mutant alkaline phosphatase (PhoA* RED ) lacking the first cysteine (C168A) and with an additional mutant cysteine (S410C in magenta) is mis-oxidized by DsbA (PhoA* MIS-OXI ), resulting in unknown disulfide bond pattern. ⑤ Colonies expressing inactive misfolded PhoA*, which cannot hydrolyze XP, remain their natural white color. ⑥ Misfolded PhoA* is isomerized by DsbC or DsbG* mutants to an active form (PhoA* OXI ) with an unknown disulfide bond pattern resulting in blue colonies. (B) Selection of DsbG* mutants on MOPS-XP plates. ① Cells lacking dsbC , dsbG , phoA and harboring an empty expression vector along with pBAD33-PhoA* are opaque and ecru when plated on the selective MOPS-XP media. ② Cells turn blue when expressing DsbC from the plasmid pDSW204. ③ Cells remain ecru when expressing DsbG from the plasmid pMER79. ④ <t>PCR</t> mutagenized library of pMER79- dsbG *-flag, transformed into cells lacking dsbC , dsbG , phoA , and blue colonies, which have active folded PhoA*, was selected. MOPS, 3-( N -morpholino)propanesulfonic acid; <t>PCR,</t> <t>polymerase</t> chain reaction; wt, wild type; XP, 5-bromo-4-chloro-3-indolyl phosphate.
Error Prone Pcr, supplied by SAMDI Tech, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/error+prone+pcr/pm33174742-59-22-1?v=SAMDI+Tech
Average 90 stars, based on 1 article reviews
error-prone pcr - by Bioz Stars, 2026-08
90/100 stars
  Buy from Supplier
error-prone pcr  (Promega)
90
Promega error-prone pcr
Principle of in vivo disulfide bond isomerase screen using mutant PhoA*. (A) ① Reduced wt alkaline phosphatase (wt PhoA RED ) with four cysteines ( yellow balls with numbers indicating cysteine residue) is correctly oxidized by DsbA to form two consecutive disulfide bonds. ② Active folded PhoA (wt PhoA OXI ) can hydrolyze XP, ③ resulting in blue colonies. ④ Reduced mutant alkaline phosphatase (PhoA* RED ) lacking the first cysteine (C168A) and with an additional mutant cysteine (S410C in magenta) is mis-oxidized by DsbA (PhoA* MIS-OXI ), resulting in unknown disulfide bond pattern. ⑤ Colonies expressing inactive misfolded PhoA*, which cannot hydrolyze XP, remain their natural white color. ⑥ Misfolded PhoA* is isomerized by DsbC or DsbG* mutants to an active form (PhoA* OXI ) with an unknown disulfide bond pattern resulting in blue colonies. (B) Selection of DsbG* mutants on MOPS-XP plates. ① Cells lacking dsbC , dsbG , phoA and harboring an empty expression vector along with pBAD33-PhoA* are opaque and ecru when plated on the selective MOPS-XP media. ② Cells turn blue when expressing DsbC from the plasmid pDSW204. ③ Cells remain ecru when expressing DsbG from the plasmid pMER79. ④ <t>PCR</t> mutagenized library of pMER79- dsbG *-flag, transformed into cells lacking dsbC , dsbG , phoA , and blue colonies, which have active folded PhoA*, was selected. MOPS, 3-( N -morpholino)propanesulfonic acid; <t>PCR,</t> <t>polymerase</t> chain reaction; wt, wild type; XP, 5-bromo-4-chloro-3-indolyl phosphate.
Error Prone Pcr, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/error+prone+pcr/pm22982168-33-5-9?v=Promega
Average 90 stars, based on 1 article reviews
error-prone pcr - by Bioz Stars, 2026-08
90/100 stars
  Buy from Supplier
error prone pcr eppcr  (Molecular Dynamics Inc)
86
Molecular Dynamics Inc error prone pcr eppcr
Principle of in vivo disulfide bond isomerase screen using mutant PhoA*. (A) ① Reduced wt alkaline phosphatase (wt PhoA RED ) with four cysteines ( yellow balls with numbers indicating cysteine residue) is correctly oxidized by DsbA to form two consecutive disulfide bonds. ② Active folded PhoA (wt PhoA OXI ) can hydrolyze XP, ③ resulting in blue colonies. ④ Reduced mutant alkaline phosphatase (PhoA* RED ) lacking the first cysteine (C168A) and with an additional mutant cysteine (S410C in magenta) is mis-oxidized by DsbA (PhoA* MIS-OXI ), resulting in unknown disulfide bond pattern. ⑤ Colonies expressing inactive misfolded PhoA*, which cannot hydrolyze XP, remain their natural white color. ⑥ Misfolded PhoA* is isomerized by DsbC or DsbG* mutants to an active form (PhoA* OXI ) with an unknown disulfide bond pattern resulting in blue colonies. (B) Selection of DsbG* mutants on MOPS-XP plates. ① Cells lacking dsbC , dsbG , phoA and harboring an empty expression vector along with pBAD33-PhoA* are opaque and ecru when plated on the selective MOPS-XP media. ② Cells turn blue when expressing DsbC from the plasmid pDSW204. ③ Cells remain ecru when expressing DsbG from the plasmid pMER79. ④ <t>PCR</t> mutagenized library of pMER79- dsbG *-flag, transformed into cells lacking dsbC , dsbG , phoA , and blue colonies, which have active folded PhoA*, was selected. MOPS, 3-( N -morpholino)propanesulfonic acid; <t>PCR,</t> <t>polymerase</t> chain reaction; wt, wild type; XP, 5-bromo-4-chloro-3-indolyl phosphate.
Error Prone Pcr Eppcr, supplied by Molecular Dynamics Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/error+prone+pcr/pm42105045-239-38-88?v=Molecular+Dynamics+Inc
Average 86 stars, based on 1 article reviews
error prone pcr eppcr - by Bioz Stars, 2026-08
86/100 stars
  Buy from Supplier

Image Search Results


Principle of in vivo disulfide bond isomerase screen using mutant PhoA*. (A) ① Reduced wt alkaline phosphatase (wt PhoA RED ) with four cysteines ( yellow balls with numbers indicating cysteine residue) is correctly oxidized by DsbA to form two consecutive disulfide bonds. ② Active folded PhoA (wt PhoA OXI ) can hydrolyze XP, ③ resulting in blue colonies. ④ Reduced mutant alkaline phosphatase (PhoA* RED ) lacking the first cysteine (C168A) and with an additional mutant cysteine (S410C in magenta) is mis-oxidized by DsbA (PhoA* MIS-OXI ), resulting in unknown disulfide bond pattern. ⑤ Colonies expressing inactive misfolded PhoA*, which cannot hydrolyze XP, remain their natural white color. ⑥ Misfolded PhoA* is isomerized by DsbC or DsbG* mutants to an active form (PhoA* OXI ) with an unknown disulfide bond pattern resulting in blue colonies. (B) Selection of DsbG* mutants on MOPS-XP plates. ① Cells lacking dsbC , dsbG , phoA and harboring an empty expression vector along with pBAD33-PhoA* are opaque and ecru when plated on the selective MOPS-XP media. ② Cells turn blue when expressing DsbC from the plasmid pDSW204. ③ Cells remain ecru when expressing DsbG from the plasmid pMER79. ④ PCR mutagenized library of pMER79- dsbG *-flag, transformed into cells lacking dsbC , dsbG , phoA , and blue colonies, which have active folded PhoA*, was selected. MOPS, 3-( N -morpholino)propanesulfonic acid; PCR, polymerase chain reaction; wt, wild type; XP, 5-bromo-4-chloro-3-indolyl phosphate.

Journal: Antioxidants & Redox Signaling

Article Title: Converting a Sulfenic Acid Reductase into a Disulfide Bond Isomerase

doi: 10.1089/ars.2014.6235

Figure Lengend Snippet: Principle of in vivo disulfide bond isomerase screen using mutant PhoA*. (A) ① Reduced wt alkaline phosphatase (wt PhoA RED ) with four cysteines ( yellow balls with numbers indicating cysteine residue) is correctly oxidized by DsbA to form two consecutive disulfide bonds. ② Active folded PhoA (wt PhoA OXI ) can hydrolyze XP, ③ resulting in blue colonies. ④ Reduced mutant alkaline phosphatase (PhoA* RED ) lacking the first cysteine (C168A) and with an additional mutant cysteine (S410C in magenta) is mis-oxidized by DsbA (PhoA* MIS-OXI ), resulting in unknown disulfide bond pattern. ⑤ Colonies expressing inactive misfolded PhoA*, which cannot hydrolyze XP, remain their natural white color. ⑥ Misfolded PhoA* is isomerized by DsbC or DsbG* mutants to an active form (PhoA* OXI ) with an unknown disulfide bond pattern resulting in blue colonies. (B) Selection of DsbG* mutants on MOPS-XP plates. ① Cells lacking dsbC , dsbG , phoA and harboring an empty expression vector along with pBAD33-PhoA* are opaque and ecru when plated on the selective MOPS-XP media. ② Cells turn blue when expressing DsbC from the plasmid pDSW204. ③ Cells remain ecru when expressing DsbG from the plasmid pMER79. ④ PCR mutagenized library of pMER79- dsbG *-flag, transformed into cells lacking dsbC , dsbG , phoA , and blue colonies, which have active folded PhoA*, was selected. MOPS, 3-( N -morpholino)propanesulfonic acid; PCR, polymerase chain reaction; wt, wild type; XP, 5-bromo-4-chloro-3-indolyl phosphate.

Article Snippet: We utilized the plasmid pMER79- dsbG -flag to construct a polymerase chain reaction (PCR) mutagenized library of dsbG*s protein-coding region using error prone PCR (GenScript).

Techniques: In Vivo, Mutagenesis, Residue, Expressing, Selection, Plasmid Preparation, Transformation Assay, Polymerase Chain Reaction